Chikwata che Yakakwirira Kanzuru yeSainzi Kuferefeta (CSIC), kuburikidza neInstitute of Advanced Chemistry of Catalonia (IQAC), yakagadzira monoclonal antibody inokwanisa kuderedza chepfu imwe yebhakitiriya ine njodzi zvikuru muchipatara inonyanya kunetseka neWorld Health Organization: Pseudomonas aeruginosaUtachiona uhwu, hunowanikwa zvakanyanya munzvimbo dzehutano, hwakazivikanwa se "superbug" nekuda kwekugona kwahwo kuramba mishonga inorwisa mabhakitiriya yakawanda iripo.
Antibody itsva, inonzi mAb122Hazvitsvage kuparadza tupukanana zvakananga, asi vhara pyocyaninchepfu huru inopedza simba rekudzivirira kwemurwere uye inokurudzira hutachiona hwakakomba. Nzira iyi, inozivikanwa senzira yekurwisa hutachiona, iri kutariswa senzira inogona kushandiswa kuderedza kushandiswa kwemishonga inorwisa mabhakitiriya mabhuku ekare uye kuderedza kubuda kwekusagadzikana kutsva, dambudziko rave richikonzera nyevero muzvipatara muSpain nemuEurope yese kwemakore.
Bakiteria yakakosheswa neWHO uye shanduko muhurongwa
La Pseudomonas aeruginosa Yakarongwa neWHO seimwe yezvirwere zvinotapukira zvakanyanya pasi rose nekuda kwe kugona kunoshamisa kwekugadzirisa uye zvinogona kukonzera kusakwanisa kushandisa mishonga yakawanda. Izvi zvine chekuita nehutachiona hwekufema, hweweti, kana hwemaronda ekuvhiyiwa, kunyanya kuvanhu vane masoja emuviri asina simba kana avo vari muchipatara kwenguva yakareba.
Muchiitiko ichi, chepfu pyocyanin inoita basa guru: iyi molecule zvinokuvadza masero emuviri wemuviriZvinochinja maitiro ekuzvimba uye zvinoita kuti hutachiona hurambe huripo uye huchikura. Saka kuderedza chepfu iyi inzira isina kunanga yekuderedza simba rebhakitiriya pasina kuirwisa zvakananga, chimwe chinhu chinoitwa nemishonga yechinyakare inorwisa mabhakitiriya uye iyo, nekufamba kwenguva, inokurudzira kusarudzwa kwemarudzi anoramba kurwisa.
Muongorori kubva kuIQAC-CSIC Lluïsa Vilaplana Yeuka kuti kugona kukuru kwezvipembenene izvi zvinoita kuti zvive zvakakosha "kukurudzira nzira itsva dzekurapa" dzinobvumira kuderedza mhando dzezvirwere zvinotsungirira zvakawanda uye kuderedza kufambira mberi kwehutachiona. Izvi zvinonyanya kuoneka mumakamuri ekuchengetwa kwevarwere vane chirwere chepfungwa (intensive care) nedzimwe nzvimbo dzezvipatara uko varwere vasina dziviriro vanowanikwa vakawanda.
Basa, rakabudiswa mujenari ACS Pharmacology neSainzi Yekushandura, inokodzera zvakakwana mukuchinja uku kwemaonero: nzira ye kurwisa hutachiona Chinangwa hachisi chekubvisa mabhakitiriya, asi kudzima zvombo zvawo zvikuru zvekurwisa. Izvi zvinoderedza kumanikidzwa kwekusarudza kunobatanidzwa nekushandiswa zvakanyanya kwemishonga inorwisa mabhakitiriya, inova imwe yemasimba ari kukonzera dambudziko rekusakwanisa kurwisa mishonga inorwisa mabhakitiriya pasi rose.
Mashandiro anoita monoclonal antibody mAb122
Boka reNanobiotechnology for Diagnosis reIQAC-CSIC rakagadzira mienzaniso yembeva yekuedza imwe antibody ye monoclonal, inonzi mAb122Monoclonal antibody inodzivirira muviri mapuroteni anogadzirwa murabhoritari iyo inoziva nemazvo molekyuru imwe chete; muchiitiko ichi, pyocyanin. Kunyatsoita uku kunobvumira kuvharirwa kwakasarudzika kwemhedzisiro yayo ine chepfu.
Kana yangowanikwa, antibody yakaedzwa pa tsika dzemacrophagemAb122, rudzi rwakakosha rwemasero ekudzivirira muviri, rwakanga rwakatarisana nehuwandu hwakasiyana hwechepfu yebhakitiriya. Zvakabuda zvinoratidza kuti mAb122 inoderedza kukuvara kwemasero inokonzerwa nepyocyanin uye inovandudza zvakanyanya kupona kwemasero aya ekudzivirira.
Uyezve, kana antibody yakapihwa pasina chepfu, hapana migumisiro ine chepfu yakaonekwa Mhedzisiro iyi inokonzerwa ne mAb122 pachayo, chinhu chakakosha pakuedza kwemangwana kwe preclinical uye kliniki. Kushaikwa kwechepfu uku ndechimwe chezvinhu zvakakosha usati waenderera mberi nekudzidza mumhando dzemhuka dzakakwana uye, mushure mezvo, muvanhu.
Muongorori Pilar MarcoMukuru wechikwata chakatungamira chidzidzo ichi anosimbirira kuti pfungwa yacho haisi yekuuraya mabhakitiriya, asi dzima mashandiro ayo ehutachionaNeku "bvisa zvombo" nenzira iyi, murwere anodzivirirwa uye, panguva imwe chete, kumanikidzwa kwakasimba kwekushanduka-shanduka kunokonzerwa nemishonga inorwisa mabhakitiriya yakawanda, uko mhando dzinorwisa zvakanyanya, dzinoramba dziripo.
Mabhenefiti ekurwisa hutachiona pane ekare emishonga inorwisa mabhakitiriya
Mishonga yekurwisa hutachiona, yakaita seiya yakakurudzirwa ne antibody ye mAb122Zvinosiyana nekurapa kwakajairika nekuti Haarwisi kugara kwenguva refu kwebhakitiriya.Pane kudaro, vanonanga zvinhu zvinokonzera hutachiona, muchiitiko ichi chepfu yepyocyanin. Nekusaedza kubvisa tupukanana, chikonzero chebhayoloji chinoita kuti mabhakitiriya agadzire shanduko dzinoadzivirira kubva kumushonga chinodzikira.
Nzira iyi ine zvakawanda mikana yekubatsira kwekirinikiKune rumwe rutivi, zvinogona kubvumira mishonga inorwisa mabhakitiriya kushandiswa chete kana zvichidikanwa, kana kuti mishonga yakadereraIzvi zvinoderedza njodzi dzinosanganisirwa nekurapwa kwenguva refu uye kwakaoma. Uyezve, zvinoderedza mukana wekugadzira mishonga mitsva. mhando dzisingapindi mishonga yakawanda, chimwe chezvinhu zvinotyisa zvikuru pahutano hwevanhu muEurope.
Muchokwadi, kurapwa kwakavakirwa pa mAb122 kunoonekwa senzira yekurapa chinopindirana chishandiso pane kutsiva mishonga inorwisa mabhakitiriya zvachose. Chinangwa chaizova chekudzivirira masero ekudzivirira chirwere uye kudzora hutachiona, zvichipa murwere nguva yekudzivirira chirwere—uye, kana zvichidikanwa, mimwe mishonga—yekutarisira mamiriro ekurapa.
Vanyori vekudzidza uku vanosimbisa kuti rudzi urwu rwekurapa runogona kubatsira zvikuru mu varwere vari muchipatara nenjodzi huru yekutapukirwa ne Pseudomonas aeruginosa, sevanhu vane zvirwere zvekufema zvisingaperi, varwere vekenza, kana vanhu vari kuvhiyiwa zvakaoma.
Mhedzisiro pamhinduro yekuzvimba uye matanho anotevera
Pamusoro pekuongorora kukuvara kwakananga kwemasero emuviri, timu yeIQAC-CSIC yakaongorora kuti antibody iyi yakaakanganisa sei. mAb122 kune mhinduro yekuzvimbaPyocyanin inochinja kugadzirwa kwema cytokine akasiyana-siyana, mamorekuru anodzora kutaurirana pakati pemasero emuviri wekudzivirira muviri uye kusimba kwekuzvimba.
Miedzo yakaratidza kuti ma antibodies yakagadzirisa mamwe emazinga aya ye cytokines, zvichiratidza kuti kudzivirira chepfu iyi kunogona kukanganisa zvikuru mashandiro anoita muviri pakubata kuzvimba panguva yehutachiona. Zvisinei, vaongorori vanosimbisa kuti zvimwe zvidzidzo zvinodiwa kuti vanzwisise zvizere kusiyana uku uye kuona kana zvichikwanisika kugadzirisa mhinduro iyi yekuzvimba nenzira inobatsira.
Parizvino, mhedzisiro yakawanikwa iri muchikamu ezvokunambira kunze kwomuviri waamaiKureva, mumasero emhuka uye murabhoritari yekuedza. Danho rinotevera richave rekushandura nzira iyi kuenda kuzvidzidzo mumhuka dziri mumuviri, zvakakosha kuti paonekwe kuchengetedzeka uye kushanda kweantibody muzvinhu zvipenyu zvose tisati tafunga nezvekuedzwa kwevanhu.
Kana data racho rikasimbiswa, nzira ye mAb122 inogona kusimbiswa senzira yekudzivirira nzira yekurapa inopindirana tichitarisana nehutachiona hunokonzerwa nemabhakitiriya asingaurayiwi nemishonga yakawanda, chimwe chinhu chakakosha kune masisitimu ehutano ekuEurope, ayo ave achinyevera kwemakore nezvekuwedzera kwezvirwere zvisingaurayiwi muzvipatara.
Nekuvandudzwa uku, boka reNanobiotechnology for Diagnostics kuIQAC-CSIC rinopa chimwe chikamu mupuzzle mukurwisa superbugs: a antibody yemonoclonal yakagadzirwa muSpain izvo, nekubvisa chepfu yepyocyanin ye Pseudomonas aeruginosaChinangwa chayo ndechekusimbisa dziviriro yemurwere, kubvumira kushandiswa kwemishonga inorwisa mabhakitiriya zvine musoro, uye kuvhura mukana wekurapa kwakananga uye kwakachengeteka kudzivirira hutachiona huri kukonzera musoro wakakomba muzvipatara parizvino.